2 edition of Experimental observations of the pathobiology of Tetrahymena (Protozoa : Ciliata) parasites in mosquito larvae found in the catalog.
Experimental observations of the pathobiology of Tetrahymena (Protozoa : Ciliata) parasites in mosquito larvae
Roger A. LeBrun
Written in English
|Statement||by Roger A. LeBrun|
|The Physical Object|
|Pagination||xvi, 166 leaves :|
|Number of Pages||166|
Many observations suggest the potential benefits of the Tetrahymena genome sequence for investigating human neurobiology. Tetrahymena has opioid receptors with pharmacological properties similar to human ones, and is already being used as a model to test natural marine compounds that inhibit pain and inflammation. This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology.Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field. With cutting edge material,this Author: Elsevier Science.
This indicated that cilia formation in Tetrahymena was dependent on protein synthesis after amputation. Conversely, colchicine was found to inhibit cilia regeneration without affecting either RNA or protein synthesis. This observation suggested the action of colchicine to be an interference with the assembly of ciliary subunit by: Based on your careful observations, you will collect some data to quantify Tetrahymena phagocytosis in the presence of India ink. A. Observations of Tetrahymena pyriformis Exposed to India Ink In a microcentrifuge tube, add 50 μL of 1% ink solution and then add 50 μL of live Tetrahymena.
Analysis of Tetrahymena and Phagocytosis Worksheet 2. Experimental question: How long does it take Tetrahymena pyriformis to ingest food? Experimental design: Determine the time it takes for Tetrahymena sp. to ingest 50% maximum food consumption. ‘Phagocytosis Time’ for 50% ingestion (or ‘PT 50 ’). Tetrahymena as a Model Organism Tetrahymena. Tetrahymena thermophila is a useful model organism for molecular research at all levels. A ciliated single-celled protozoan, Tetrahymena is a freshwater organism that inhabits streams, lakes, and ponds and can be found almost everywhere, in a range of climates.
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Tetrahymena emerged as an experimental organism largely due to its interesting biology. However, its utility for exploring many diverse scientific questions is greatly facilitated by the availability of many tools for molecular genetic analysis and strain engineering. Tetrahymena thermophila is a powerful experimental organism that combines a biological complexity similar to that of metazoans (including mammals) with the experimental accessibility of a protist.
Past investigations with Tetrahymena have yielded several key discoveries, including dynein, catalytic RNA, and telomerase.4/5(1). The Tetrahymena Stock Center is supported by NIH grant # P40 RRA1.
DCH is supported by NIH NCRR SEPA grant 5 R25 RR and by NIH grant # P40 RRA1. The Ciliate Genomics Consortium is funded by NSF CAREER grant # to EAW. DCH is a founding member of Tetragenetics, Inc., Ithaca, NY, Cited by: 7. Observe and document Tetrahymena behavior with and without the 9 volt battery D.
Experiments with Aerotaxis in Tetrahymena Introduction Aerotaxis in Tetrahymena is when the organism moves toward an O 2-rich environment. It is a dramatic occurrence in Tetrahymena, but not much is known about its underlying mechanism.
Experiments Size: KB. 14 Donna M. Bozzone, Investigating Phagocytosis in Tetrahymena, The American Biology Teacher,62, 2, CrossRef; 15 Donna M. Bozzone, Investigating Phagocytosis in Tetrahymena: An Experimental System Suitable for Introductory & Advanced Instruction, The American Biology Teacher,62, 2, CrossRef.
Tetrahymena thermophila is a ciliate -- a unicellular eukaryote. Remarkably, every cell maintains differentiated germline and somatic genomes: one silent, the other expressed. Moreover, the two genomes undergo diverse processes, some as extreme as life and death, simultaneously in the same ved eukaryotic mechanisms have been modified in ciliates to.
Tetrahymena pyriformis is a ciliated protist that can be used to investigate the effects of cigarette extracts on ciliary function. The cilia lining human lungs and those on the surface of Tetrahymena are structurally similar.
In Tetrahymena, rows of cilia cover the surface of the cell. The integrated, alternate bending of the cilia creates the. Dynamics of the sex ratio in Tetrahymena thermophila.
it is found that the experimental observations highly support theoretical predictions. Certain to become the defining book. Tetrahymena's versatile biology provides extremely useful experimental tools. With a minimum doubling time below 2 hr, it is among the fastest growing eukaryotes.
It readily grows to high density over a wide range of scale (from a few ul to fermentor scale) in a variety of media: totally defined synthetic chemical medium, rich nutrient broth or monobacterial culture. Clinical Investigation The pathobiology of subclinical pyelonephritisâ€”An experimental evaluation Thomas E.
Miller, 1 * Glenne Findon, 1 Stephen P. Rainer, 1 John B. Gavin, 1 1 Departments of Medicine and Pathology, University of Auckland, Auckland, New Zealand Departments of Medicine and Pathology, University of Auckland Auckland New Zealand * Cited by: 6.
the fact remains that experimental evidence for a dual capacity for nutrient uptake in T. pyriformis was presented more than 50 years ago. Few investigators have compared the contribu- tion of the food vacuoles to total nutrient up- take of T.
pyriformis (LWOFF (55), SEAMAN (98,Cited by: Tetrahymena has been used as a model cell system in many studies of morphogenesis, conjugation, gene mapping, cell division and growth kinetics. Uptake of particles by the ciliate Tetrahymena.
In C.L. Harris (Ed.), Tested Studies for Labora- tory Teaching. Proceedings of the Fourth Workshop/Conference of the Association for Biology Laboratory Edu- cation (ABLE). Phagocytosis in Tetrahymena Procedure We were given regular and starved tetrahymena on a microscope slide, then placed 5 drops of 1% India ink onto the microscope slide with a micropipette, this ink helps us with the visualization of the.
Phagocytosis in Tetrahymena as an Experimental System To Study the Toxic Effects of Cigarette Smoke Preparation for Laboratory: Web Tutorial 6, Tetrahymena - submit questions Additional Background: Freeman - sections on microtubules and cilia, pp Background Tetrahymena are small unicellular organisms that can be found in pond water File Size: KB.
Abstract. Those who study ciliates have struggled over the years to establish a place in the pantheon of model organisms. 1 Eukaryotic cell biologists have turned profitably to yeast models for the powerful genetic tools at their disposal, while developmental biologists have cultivated a gallery of metazoan embryos with contributions from the plant and fungal by: The organism’s biochemical genetics, vitamin and inorganic requirements, and evolution are also shown.
This book also explores the effect of radiation, drugs, and hydrostatic pressure on Tetrahymena. The documented information presented in this book will be sufficient to stimulate even more interest in the Edition: 1. Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics.
The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to 4/5(1).
Tetrahymena thermophila is a ciliate with hundreds of cilia primarily used for cellular motility. These cells propel themselves by generating hydrodynamic forces through coordinated ciliary beating.
The coordination of cilia is ensured by the polarized organization of basal bodies (BBs), which exhibit remarkable structural and molecular conservation with BBs Cited by: Search term.
Advanced Search Citation Search. Search term. As a ciliated protozoan, Tetrahymena thermophila exhibits nuclear dimorphism: two types of cell nuclei.
They have a bigger, non-germline macronucleus and a small, germline micronucleus in each cell at the same time and they both carry out different functions with distinct cytological and biological : Oligohymenophorea.Tetrahymena is able to adapt to the presence of sublethal concentrations of many drugs which inhibit a wide variety of cellular functions.
In spite of the generality of this phenomenon in Tetrahymena, the mechanism of adaptation at the cellular and molecular levels is study deals mainly with adaptation to the protein synthesis inhibitors, cycloheximide and by: Drugs and the Cell Cycle provides an introduction to fundamental principles and studies on the mechanisms of drug action on proliferating cells.
The book is intended to reduce the time lag between observation and practical Edition: 1.